DTNB CAS 69-78-3

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DTNB (5,5 '- dithiobis (2-nitrobenzoic acid), CAS: 69-78-3), Also known as Elman reagent, it is a classic and indispensable thiol quantification analysis reagent in the field of biochemistry. Its core function is to undergo specific and sensitive chemical reactions with free thiol groups, generating yellow 5-mercapto-2-nitrobenzoic acid products, thereby achieving accurate and convenient determination of thiol content in biological samples.
The mechanism of action of this reagent is efficient and direct. One molecule of DTNB can undergo a reduction reaction with two thiol groups, breaking its own disulfide bond. At the same time, for every thiol group consumed, a yellow anion with strong absorption at a wavelength of 412nm is released. This quantitative relationship with a molar ratio of 1:2 and significant changes in optical signals make it one of the most reliable methods in quantitative analysis. Its high sensitivity allows for accurate detection of trace samples, with applications ranging from purified proteins to complex biological tissue fluids.
In practical applications, the value of DTNB is reflected in multiple key research areas. It is widely used to determine the concentration of low molecular weight thiols such as glutathione and cysteine, and is an important tool for evaluating cellular antioxidant status. It is specifically applied to determine the glutathione content in agricultural products such as goji berries. At the same time, it is also the gold standard method for studying the content and function of thiol rich protein molecules such as metallothionein. By quantifying thiol groups in biological samples, researchers can delve into many core biological processes such as oxidative stress, metal metabolism, protein structure and function.

 

 

 

 

    

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